Using the micronucleus assay to detect genotoxic effects of metal ions.
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چکیده
منابع مشابه
Using the micronucleus assay to detect genotoxic effects of metal ions.
The lymphocyte micronucleus assay was used to measure the average frequency of micronuclei in a population and thus assess genotoxic effects. Data from 174 persons give an average value of 16.4 +/- 7.3, and a slight age-dependence was observed. To detect combined environmental mutagen injuries the micronucleus assay was used to study the effects of metal compounds. Cadmium ions increased the mi...
متن کاملResearch Article: Determination of genotoxic effects of aluminum on Cirrhinus mrigala and Ctenopharyngodon idella using comet assay and micronucleus test
Metals are the major pollutants in aquatic environment. A lot of research literature is available globally about accumulation of heavy metals in aquatic ecosystems as well as in different body organs of aquatic animals, especially fish. Heavy metals mainly generate reactive oxygen species (ROS) in aquatic animals that lead to oxidative stress production. ROS cause oxidative damage to different ...
متن کاملGenotoxic effects of oestrogens in breast cells detected by the micronucleus assay and the Comet assay.
Cumulative exposure to oestrogen has been linked to increased risk of breast cancer. Whilst oestrogens induce cancers in rodent bioassays it is unclear whether the mechanisms involved are genotoxic and/or epigenetic. The cytokinesis block micronucleus (CBMN) and the alkaline single cell-gel electrophoresis 'Comet' assays were used to examine MCF-7 cells for chromosomal damage and DNA single-str...
متن کاملMicronucleus assay- an early diagnostic tool to assess genotoxic changes in tobacco and related habits
Objective: Micronuclei are induced in cells by a variety of substances, like UV radiation, infrared rays, X-radiations, and chemicals. Among them tobacco- specific nitrosamines have been reported to be potent mutagenic agents which are thought to be responsible for the induction of chromosomal aberrations resulting in production of micronuclei. The main aim of our study is to compare MN frequen...
متن کاملUse of the fluorescent micronucleus assay to detect the genotoxic effects of radiation and arsenic exposure in exfoliated human epithelial cells.
The exfoliated cell micronucleus (MN) assay using fluorescent in situ hybridization (FISH) with a centromeric probe is a rapid method for determining the mechanism of MN formation in epithelial tissues exposed to carcinogenic agents. Here, we describe the use of this assay to detect the presence or absence of centromeric DNA in MN induced in vivo by radiation therapy and chronic arsenic (As) in...
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ژورنال
عنوان ژورنال: Environmental Health Perspectives
سال: 1993
ISSN: 0091-6765,1552-9924
DOI: 10.1289/ehp.101-1521140